Direct immunofluorescent detection of Legionella pneumophila in respiratory specimens

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detection of legionella pneumophila in urine and serum specimens of neutropenic febrile patients with haematological malignancies

legionella pneumophila ( l. pneumophila ) is a gram-negative bacterium which causes ‎legionnaires’ disease as well pontiac fever. the legionella infections in patients suffering from ‎neutropenia- as a common complication of cancer chemotherapy- can distribute rapidly. we ‎aimed to detection of l. pneumophila in haematological malignancy suffering patients with ‎nuetropenic fever by targeting t...

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Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens.

The efficiencies of five commercially available nucleic acid extraction methods were evaluated for the recovery of a standardized inoculum of Legionella pneumophila in respiratory specimens (sputum and bronchoalveolar lavage [BAL] specimens). The concentrations of Legionella DNA recovered from sputa with the automated MagNA Pure (526,200 CFU/ml) and NucliSens (171,800 CFU/ml) extractors were gr...

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Enhancement of recovery of Legionella pneumophila from contaminated respiratory tract specimens by heat.

Heating of Legionella pneumophila and other Legionella spp. was studied to determine whether this technique could be used as a selective technique with contaminated clinical specimens. Studies of 13 different strains of Legionella spp. showed heterogeneous heat survival; heating at 60 degrees C for 1 to 2 min did not affect the survival of the majority of strains. Heating of four Pseudomonas ae...

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Investigation of Legionella Pneumophila bacteria in hospital water supply systems

Introduction: Legionella Pneumophila bacteria is known as one of the most important nosocomial infections and the most common cause of death in patients. This study was aimed to identify Legionella Pneumophila bacteria in hospital water supply systems. Material and Methods: This descriptive cross-sectional study was performed to identify Legionella pneumophila in hot and cold-water systems of i...

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Detection of Legionella Pneumophila in Urine and Serum Specimens of Neutropenic Febrile Patients with Haematological Malignancies

Background: Legionella pneumophila (L. pneumophila) is a gram-negative bacterium which causes ‎Legionnaires' disease as well as Pontiac fever. The Legionella infections in patients suffering from ‎neutropenia- as a common complication of cancer chemotherapy- can distribute rapidly. We ‎aimed to detect of L. pneumophila in haematological malignancy suffering patients with ‎neutropenic fever by t...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1980

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.11.1.59-64.1980